Table 1.
Summary of YAC → cosmid MCD maps for portions of human chromosome 7
| Chromosome 7 YACs | Coverage* | Nf† (EcoRI) | Nf† (HindIII) | Nf† (NsiI) | Coligations,‡ % | Map size,§ kbp |
|---|---|---|---|---|---|---|
| yWSS771 | 30.3 | 9.8/1.2 | 8.4/1.2 | 11.4/1.2 | 2.8 | 44 + 170 |
| yWSS1346 | 29.2 | 10.5/1.2 | 12.4/1.3 | 10.0/1.3 | 3.0 | 281 |
| yWSS1434 | 20.5 | 7.4/1.3 | 6.8/1.4 | 7.4/1.6 | 7.8 | 156 |
| yWSS1564 | 16.7 | 9.2/1.3 | 10.4/1.5 | 9.8/1.3 | 7.9 | 640 |
| yWSS1572 | 31.5 | 8.0/1.2 | 9.1/1.2 | 9.0/1.3 | 4.5 | 292 |
| yWSS1613 | 26.3 | 10.6/1.2 | 10.6/1.1 | 11.5/1.3 | 3.5 | 136 + 56 |
| yWSS1862 | 23.4 | 8.4/1.2 | 11.0/1.2 | 11.6/1.3 | 3.4 | 261 |
| yWSS1980 | 20.7 | 8.3/1.1 | 8.5/1.1 | 10.8/1.1 | 5.7 | 278 |
Coverage is calculated assuming a 40-kbp insert size. Clones left out of the map because they could not be uniquely placed are included in this calculation; coligations and yeast impurities are not.
Nf refers to the average number of fragments observed in a clone, which is the first number given in each row. The second number indicates the average number of fragments per fragment group, an indication of how well ordered the restriction fragments are in the maps. Contigs smaller than 100 kbp are not included when summarizing fragments per fragment group.
Coligations are cosmids that contain a human insert from the targeted region and an unrelated piece of DNA that is inserted between the end of the human insert and the cosmid vector.
Map sizes are based on the sum of the restriction fragment sizes. The gap in the overlap region between YACs yWSS771 and yWSS1613 has not yet been closed. These two maps agree perfectly on either side of the gap and stop abruptly at the same fragments at the gap.