Figure 2.

Detection of HLA-G transcripts in the K562 parental cell line, transfectants, and the JEG-3 cell line. Reversed RNAs were amplified using G.257 and G.1225 HLA-G-specific primers and Southern blot was hybridized with G.1200 or G.526 ³²P-labeled probe. Positive (+) and negative (−) lanes correspond to the RT⁺ and RT⁻ templates, and the blank is a control performed using PCR mixture without the cDNA template, as described. To control the amount of RNA in all samples, RT-PCR amplification results obtained with β-actin-specific primers and Southern blots were hybridized with β-actin ³²P-labeled probe.