Figure 3.

Immunoprecipitation, SDS/PAGE, and Western blot analysis of biotin-labeled cell surface proteins. Biotinylated membrane lysates from K562 (lanes 1 and 2), K562-pRc/RSV (lanes 3 and 4), K562-HLA-G1 (lanes 5 and 6), K562-HLA-G2 (lanes 7 and 8), and JEG-3 (lanes 9 and 10) were immunoprecipitated with control mAb UPC-10 (lanes 1, 3, 5, 7, and 9) or with mAb W6/32 (lanes 2, 4, 6, 8, and 10) and analyzed by 10% SDS/PAGE under reducing conditions. Size markers in kDa are indicated at the right.