Figure 4.
Expression of HLA molecules on the transfectants of the K562 cell line detected by cytofluorometry. Wild-type K562 cells (K562), K562 cells transfected with either the vector alone (K562 pRc/RSV), HLA-G1 (K562-HLA-G1) or with HLA-G2 (K562-HLA-G2), and JEG-3 cells were labeled by indirect immunofluorescence with the following primary mAbs (bold profiles): W6/32 (monomorphic anti-class I) (Left) and B1.G6 (anti-human β2m) (Right). Controls were the same cells stained with an isotype-matched control antibody (light profiles). After washing, cells were stained with phycoerythrin-conjugated F(ab′)2 goat anti-mouse IgG. One of four representative experiments is shown.