Figure 2.

(A) Schematic depiction of the main ORF in the IL-16 cDNA. The horizontal bar represents the originally published ORF of 390 bp (10). The shaded region represents the subsequently reported IL-16 ORF (14, 15). The locations of oligonucleotides used in RACE experiments are shown as arrows. The triangle identifies a splice junction at nucleotide position 129 where frequently short intervening sequences of variable length (80–200 bp) have been found. The putative initiation codon is shown as a black vertical bar. (B) Heterogenous starting points for the initiation of transcription of the IL-16 gene. Only the very beginning of the IL-16 cDNA 5′ end as found in different clones is shown. Clones 1–4 were obtained using parts of the CapFinder system. Clones 5–7 were generated by the tailing strategy. All sequence numbering in this report relates to the first base of clone 3 as being nucleotide 1.