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Immunoblot detection of pro-IL-16 in cell lysates. Lysates from 2.5 × 106 cells were loaded into each lane. Lane 1, normal COS-7 cells; lane 2, pro-IL-16 cDNA transfected COS-7 cells; lane 3, phytohemagglutinin/IL-2 stimulated PBMCs; lane 4, PBMCs after 24 hr of stimulation with 10 μM serotonin.
