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Proteolytic cleavage of rIL-16His in lysates of CD8(+) cells. Lane 1, rIL-16His in lysate after 0 min incubation; lane 2, rIL-16His6 in lysate after a 24 hr incubation; lane 3, lysate without added protein; lane 4, rIL-16His6 after a 24 hr incubation in identical buffer conditions but no added lysate. Note the aberrant migration behavior of rIL-16His, which has a calculated molecular mass of 15 kDa, and of the cleaved product.
