Figure 6.

Failure of GIF derivatives to induce cell surface TNF-α on RAW264.7 cells. RAW264.7 cells were incubated with 0 (bold solid line), 0.1 μg/ml (dashed line), 1.0 μg/ml (dashed and dotted line), or 10 μg/ml (dotted line) of LPS (a), rGIF (b), C57A/N106S (c), or C57A-DTNB (d) for 12 hr. Cell-surface TNF-α was stained and analyzed by flow cytometry. The thin solid line represents background immunofluorescence. The ordinate and abscissa represent the cell number and the fluorescence intensity on a logarithmic scale, respectively. Data presented are representative of comparable results from three experiments.