Table 1.

Comparison of reversion rates of different Vn constructs in NSO and 18.81 cells

Transfectant	Sequence	Rate, bp/generation
NSO/Vn38C-T	TGG GTC TAG CAG AGG	6.64 × 10−4
Subclones
3		6.83 × 10−4
17		6.49 × 10−4  5.93 × 10−4
21		4.46 × 10−4
NSO/Vn38A-T	TGG GTC TAG AAG AGG	3.44 × 10−5
Subclones
4		3.62 × 10−5
6		2.06 × 10−5  2.37 × 10−5
16		1.44 × 10−5
18.81/Vn38C-T	TGG GTC TAG CAG AGG	1.52 × 10−5
18.81/Vn38A-T	TGG GTC TAG AAG AGG	9.10 × 10−7
NSO/Vn94C-T	GCC GTC TAG CAC TGT	3.75 × 10−5
Subclones
6		3.79 × 10−5
13		6.15 × 10−6  2.26 × 10−5
16		2.38 × 10−5
NSO/Vn94A-T	GCC GTC TAG AAC TGT	<1.11 × 10−6
Subclones
1		7.88 × 10−7
4		4.24 × 10−7  7.80 × 10−7
11		1.13 × 10−6
NSO/Vn50A-T1	ATT GTC TAG ATT GAT	3.73 × 10−6
NSO/Vn50A-T2		1.84 × 10−6
Subclones
8		2.41 × 10−6
13		3.22 × 10−6  2.58 × 10−6
18		2.11 × 10−6
NSO/Vn65A-T1	AAG TTC TAG AGC AAG	6.49 × 10−4
Subclones
1		6.41 × 10−4
2		8.21 × 10−4  5.96 × 10−4
3		3.25 × 10−4
NSO/Vn65A-T2		5.36 × 10−4
Subclones
7		5.78 × 10−4
21		6.49 × 10−4  6.15 × 10−4

P < 0.001 for both Vn38C/Vn38A and Vn94C/Vn94A. RGYW motifs are underlined.