Figure 4.

EtxB alters the nature of the T cell response to CII. Inguinal lymph node cells, taken from mice 15 days after challenge with either CII or CII plus EtxB, were cultured in the presence or absence of 50 μg/ml CII. On each day of lymph node cell culture, triplicate samples were removed and assayed for the production of IFN-γ or IL-4 over an 18-h period by standard ELISA. Cytokine levels were calculated against standard curves produced with the appropriate recombinant cytokine by weighted probit analysis. The quantities of each cytokine (as indicated) produced in lymph node cell cultures from unprotected mice in the presence (▪) or absence (□) of collagen and from protected mice in the presence (▨) or absence (▧) of collagen are shown.