Table 1.

Histological signs of joint damage in collagen- induced arthritis

Collagen*	Toxin†	Incidence‡	Histological score§
−	−	0/7 (0%)	0.00  ± 0.00
+	−	10/14 (71%)	1.88  ± 0.35
+	EtxB (day 0)	2/15 (13%)	0.62  ± 0.38
+	EtxB (day 21)	3/7 (43%)	1.62  ± 1.59
+	EtxB(G33D) (day 0)	5/7 (71%)	1.92  ± 0.49

^*Bovine CII given in CFA s.c. at the base of tail on day 0 and in IFA on day 21. 

^†Either EtxB or EtxB(G33D) (100 μg) given in IFA s.c. on the dorsal flank on the day indicated. 

^‡The number of mice with arthritis assessed histologically 45 days after collagen injection over the total group size. 

^§Sections of knee joints were scored blind for joint changes according to the following grading system: 0, normal; 1, synovial hyperplasia with pannus formation and mild inflammation or noninflammatory mild articular cartilage degeneration; 2, articular cartilage degeneration with synovial hyperplasia and pannus formation, and moderate to severe inflammation (polymorphonuclear leukocytes and macrophages); 3, Articular cartilage degeneration with synovial hyperplasia and pannus formation, severe inflammation (polymorphonuclear leukocytes and macrophages), and significant inflammation in joint space with polymorphonuclear leukocytes, macrophages, and debris. Data are shown as the mean ± SEM of the positive animals.