Table 2. Quantitative Comparison of Human Proteins Detected.
| Number of peptides identified | ||||||
| Acc# | exp.30min* | exp.2h | C30min** | C2h | C2hpunct*** | Name |
| Keratins | ||||||
| various | 487 | 866 | 360 | 815 | 869 | type 2 keratins |
| various | 302 | 406 | 194 | 460 | 596 | type 1 keratins |
| Q5DT20 | 0 | 8 | 0 | 7 | 44 | Hornerin |
| Protease inhibitors (serum and intra cellular) | ||||||
| P01023 | 15 | 52 | 117 | 161 | 102 | Alpha-2-macroglobulin precursor |
| P01009 | 24 | 39 | 56 | 83 | 57 | Alpha-1-antitrypsin precursor |
| P05155 | 2 | 0 | 15 | 15 | 7 | Plasma protease C1 inhibitor precursor |
| P01011 | 5 | 4 | 8 | 6 | 4 | Alpha-1-antichymotrypsin precursor |
| P01008 | 3 | 4 | 4 | 1 | 5 | Antithrombin-III precursor |
| P61769 | 0 | 0 | 0 | 1 | 0 | Beta-2-microglobulin precursor |
| P02760 | 0 | 0 | 0 | 2 | 1 | AMBP protein precursor [Contains: Alpha-1-microglobulin] |
| Q3SYB4 | 0 | 2 | 0 | 0 | 0 | SERPINB12 protein |
| P35237 | 0 | 1 | 0 | 0 | 0 | Serpin B6 |
| P01042 | 0 | 1 | 0 | 1 | 1 | Kininogen-1 precursor |
| P01040 | 0 | 0 | 0 | 1 | 0 | Cystatin-A |
| O75635 | 0 | 1 | 0 | 0 | 0 | Serpin B7 |
| P05543 | 0 | 0 | 1 | 0 | 0 | Thyroxine-binding globulin precursor |
| Immunoglobulins and major serum proteins | ||||||
| P02768 | 353 | 483 | 385 | 546 | 514 | Serum albumin precursor |
| P02766 | 7 | 8 | 12 | 23 | 17 | Transthyretin precursor |
| P43652 | 0 | 0 | 1 | 1 | 0 | Afamin precursor |
| Q9UL85 | 0 | 3 | 0 | 5 | 2 | Myosin-reactive immunoglobulin kappa chain variable region |
| Q6GMY2 | 0 | 3 | 0 | 10 | 4 | IGHM protein |
| P01620 | 2 | 6 | 0 | 10 | 5 | Ig kappa chain V-III region SIE |
| P01876 | 3 | 11 | 14 | 20 | 14 | Ig alpha-1 chain C region |
| Q6IN78 | 19 | 105 | 30 | 63 | 58 | IGHG1 protein also Q6PJ95 |
| Q6GMV9 | 16 | 45 | 21 | 58 | 33 | IGKC protein also Q6PIH6 |
| P68871 | 49 | 69 | 41 | 90 | 82 | Hemoglobin subunit beta |
| P69905 | 25 | 45 | 30 | 63 | 60 | Hemoglobin subunit alpha |
| P02787 | 16 | 73 | 48 | 74 | 51 | Serotransferrin precursor |
| P06727 | 0 | 1 | 7 | 4 | 6 | Apolipoprotein A-IV precursor |
| P05090 | 0 | 6 | 4 | 6 | 5 | Apolipoprotein D precursor |
| P02647 | 11 | 15 | 25 | 46 | 20 | Apolipoprotein A-I precursor |
| P02649 | 1 | 0 | 10 | 7 | 4 | Apolipoprotein E precursor |
| P00450 | 1 | 36 | 21 | 52 | 31 | Ceruloplasmin precursor |
| P00738 | 3 | 19 | 23 | 26 | 25 | Haptoglobin precursor [ Haptoglobin alpha chain; Haptoglobin beta chain] |
| P02679 | 2 | 17 | 8 | 24 | 11 | Fibrinogen gamma chain precursor |
| P02671 | 0 | 4 | 6 | 12 | 1 | Fibrinogen alpha chain precursor [Contains: Fibrinopeptide A] |
| P02675 | 0 | 6 | 4 | 10 | 8 | Fibrinogen beta chain precursor [Contains: Fibrinopeptide B] |
| Q6GTG1 | 6 | 11 | 18 | 8 | 7 | Group-specific component |
| P02753 | 0 | 2 | 3 | 3 | 1 | Plasma retinol-binding protein precursor |
| P15090 | 2 | 1 | 2 | 2 | 1 | Fatty acid-binding protein, adipocyte |
| P31151 | 2 | 0 | 0 | 0 | 0 | Protein S100-A7 |
| P02763 | 9 | 12 | 10 | 16 | 16 | Alpha-1-acid glycoprotein 1 precursor overlapping with Q5T539, Q5U067 |
| P19652 | 2 | 5 | 2 | 5 | 5 | Alpha-1-acid glycoprotein 2 precursor overlapping P02763,unique listed |
| P25311 | 2 | 8 | 5 | 5 | 3 | Zinc-alpha-2-glycoprotein precursor |
| P02790 | 4 | 4 | 2 | 6 | 6 | Hemopexin precursor |
| P04217 | 0 | 6 | 5 | 6 | 4 | Alpha-1B-glycoprotein precursor |
| P02765 | 0 | 0 | 1 | 5 | 4 | Alpha-2-HS-glycoprotein precursor |
| P04196 | 0 | 1 | 0 | 0 | 0 | Histidine-rich glycoprotein precursor |
| P02743 | 0 | 1 | 3 | 5 | 4 | Serum amyloid P-component precursor |
| P02735 | 0 | 0 | 1 | 2 | 0 | Serum amyloid A protein precursor |
| P35542 | 0 | 0 | 1 | 1 | 1 | Serum amyloid A-4 protein precursor |
| Q49AQ8 | 0 | 0 | 0 | 0 | 2 | SELENBP1 protein |
| P02144 | 0 | 2 | 4 | 9 | 2 | Myoglobin |
| Complement associated proteins | ||||||
| P01024 | 5 | 10 | 23 | 39 | 13 | Complement C3 precursor |
| P00751 | 0 | 0 | 3 | 6 | 6 | Complement factor B precursor |
| P02748 | 0 | 1 | 0 | 0 | 0 | Complement component C9 precursor |
| P07360 | 0 | 0 | 0 | 1 | 0 | Complement component C8 gamma chain precursor |
| P0C0L4 | 0 | 0 | 1 | 7 | 0 | Complement C4-A precursor |
| Q4LE82 | 0 | 1(2) | 0 | 0(7) | 0 | C4A variant protein 1 unique sequence, all others shared with P0C0L4 |
| P10909 | 1 | 0 | 4 | 2 | 3 | Clusterin precursor |
| Epidermal cell or Desmosome-associated proteins | ||||||
| P60709 | 34 | 53 | 14 | 32 | 26 | Actin, cytoplasmic 1 |
| P06396 | 0 | 9 | 1 | 2 | 4 | Gelsolin precursor |
| Q01995 | 0 | 0 | 1 | 2 | 2 | Transgelin |
| P12273 | 0 | 0 | 1 | 0 | 0 | Prolactin-inducible protein precursor |
| Q02413 | 5 | 3 | 3 | 8 | 5 | Desmoglein-1 precursor |
| P08670 | 0 | 0 | 4 | 7 | 1 | Vimentin |
| Q5D862 | 2 | 2 | 0 | 0 | 1 | Ifapsoriasin |
| Q15151 | 0 | 0 | 0 | 1 | 2 | Plakoglobin |
| P15924 | 0 | 0 | 0 | 0 | 2 | Desmoplakin also P14923 |
| Q13748 | 5 | 0 | 0 | 0 | 0 | Tubulin alpha-3C/D chain |
| Q13509 | 3 | 0 | 0 | 0 | 0 | Tubulin beta-3 chain |
| P68371 | 5 | 0 | 0 | 0 | 0 | Tubulin beta-2C chain |
| P62158 | 0 | 0 | 1 | 1 | 0 | Calmodulin |
| Basement membrane or matrix proteins | ||||||
| P51884 | 2 | 9 | 20 | 15 | 11 | Lumican precursor |
| P23142 | 0 | 0 | 1 | 4 | 3 | Fibulin-1 precursor |
| O95617 | 0 | 0 | 0 | 1 | 0 | Fibronectin |
| Monokines | ||||||
| P07333 | 0 | 0 | 0 | 1 | 0 | Macrophage colony-stimulating factor 1 receptor precursor |
| P14174 | 0 | 0 | 0 | 1 | 0 | Macrophage migration inhibitory factor |
| Cytosolic proteins | ||||||
| P08107 | 1 | 0 | 0 | 0 | 0 | Heat shock 70 kDa protein 1 |
| O75322 | 0 | 1 | 0 | 0 | 0 | Hsp89-alpha-delta-N |
| P11142 | 1(4) | 0 | 0 | 0 | 0 | Heat shock cognate 71 kDa protein 1 unique sequence for human |
| P02792 | 0 | 0 | 1 | 2 | 1 | Ferritin light chain |
| Q86SE5 | 0 | 1 | 0 | 2 | 1 | RNA-binding Raly-like protein |
| O60814 | 0 | 0 | 0 | 0 | 2 | Histone H2B type 1-K |
| P16104 | 1 | 0 | 0 | 0 | 0 | Histone H2A.x |
| P62805 | 4 | 0 | 0 | 0 | 2 | Histone H4 |
| Q14865 | 0 | 0 | 0 | 0 | 1 | AT-rich interactive domain-containing protein 5B |
| P32119 | 3 | 1 | 8 | 6 | 4 | Peroxiredoxin-2 |
| P30041 | 0 | 0 | 0 | 4 | 1 | Peroxiredoxin-6 |
| Q06830 | 0 | 0 | 2 | 0 | 0 | Peroxiredoxin-1 |
| P00441 | 0 | 0 | 0 | 2 | 0 | Superoxide dismutase [Cu-Zn] |
| P30043 | 0 | 1 | 1 | 1 | 0 | Flavin reductase |
| P62937 | 2 | 1 | 6 | 2 | 1 | Peptidyl-prolyl cis-trans isomerase A |
| P00915 | 10 | 19 | 10 | 14 | 14 | Carbonic anhydrase 1 |
| P00918 | 1 | 0 | 3 | 4 | 0 | Carbonic anhydrase 2 |
| P07451 | 0 | 2 | 1 | 1 | 0 | Carbonic anhydrase 3 |
| P06744 | 0 | 2 | 0 | 0 | 0 | Glucose-6-phosphate isomerase |
| P04406 | 0 | 1 | 1 | 1 | 0 | Glyceraldehyde-3-phosphate dehydrogenase |
| P06733 | 0 | 1 | 0 | 0 | 0 | Alpha-enolase |
| P60174 | 0 | 1(2) | 0 | 1(2) | 0 | Triosephosphate isomerase 1 unique human sequence |
| P25705 | 4 | 0 | 0 | 0 | 0 | ATP synthase subunit alpha, mitochondrial precursor |
| P40925 | 0 | 0 | 0 | 1 | 0 | Malate dehydrogenase, cytoplasmic |
| P00338 | 0 | 0 | 0 | 1 | 0 | L-lactate dehydrogenase A chain |
| P09668 | 0 | 1 | 0 | 0 | 0 | Cathepsin H precursor |
| P31944 | 0 | 1 | 1 | 1 | 0 | Caspase-14 precursor |
| P07339 | 1 | 3 | 1 | 1 | 0 | Cathepsin D precursor |
| P06732 | 0 | 0 | 1 | 1 | 0 | Creatine kinase M-type |
| P00558 | 0 | 9 | 0 | 0 | 0 | Phosphoglycerate kinase 1 some overlapping with parasite protein |
| P63104 | 0 | 0 | 1 | 0 | 0 | 14-3-3 protein zeta/delta |
| P61626 | 0 | 0 | 1 | 0 | 0 | Lysozyme C precursor |
| Q6B0J6 | 0 | 0 | 1 | 0 | 0 | Paraoxonase 1 |
All replicate identifications above the acceptance threshold were counted.
Proteins in boxes are related.
Mass spec data and peptides for Table 2 can be viewed as: Table S6, 30 min cercarial exposure, human peptides; Table S7, 2 hr cercarial exposure, human peptides; Table S8, 30 min control human peptides; Table S9, 2 hr control human peptides; and Table S10, 2 hr puncture human peptides.
*: exp: cercaria on skin.
**: C: water on skin.
***: : water on punctured skin.