Figure 3.

Flow cytometric analysis of PB, BM, and spleen cells from leukemic hCG–PML/RARα transgenic and wild-type mice. The cells were analyzed with antibodies against the cell surface markers, c-Kit, Gr-1, and Mac-1, conjugated with fluorescein isothiocyanate (Mac-1) or R-phycoerythrin (others) (solid lines), and with control antibodies of the same isotype (dotted lines). Before Gr-1 and Mac-1 staining, cells were incubated with CD16/32 antibody to block non-antigen-specific binding of antibodies to the murine FcγII/III receptors. Values given in upper right corner of each histogram indicate the positive population for each antibody.