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. 2008 Jul 17;118(8):2747–2757. doi: 10.1172/JCI35067

Figure 3. Inhibition of hCASMC proliferation with MRP4 siRNA.

Figure 3

Quantitative real-time PCR with gene-specific primers of (A) MRP4 and (B) MRP5 in hCASMCs transfected for 72 h with MRP4, MRP5, or scrambled siRNA (n = 3, ***P < 0.001). (C) Western blot analysis of total lysates of hCASMCs transfected for 72 h with MRP4 siRNA or scrambled siRNA, showing efficient silencing of MRP4. Proteins were incubated with anti-MRP4 or anti-PP2B antibodies. PP2B was used as a loading control. n = 3; P ≤ 0.001. (D) Effect of MRP4 and MRP5 siRNAs on hCASMC proliferation (assessed by BrdU incorporation) compared with scrambled siRNA. Proliferation was normalized to the value obtained with 0.1% S. Five experiments were performed in triplicate. ***P ≤ 0.001 for MRP4 compared with scrambled or MRP5 siRNA.