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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Dev Biol. 2008 Feb 26;317(1):95–105. doi: 10.1016/j.ydbio.2008.02.019

Fig. 1.

Fig. 1

Cited2 expression in developing lungs. (a) Ten-microgram total RNA isolated from E13.5–18.5 lung tissue was subjected to Northern analysis with a specific probe for Cited2. The 18S RNA levels were used as the loading control. (b) Cited2 expression was examined on lung cryosections by detecting β-galactosidase activity from the Cited2-lacZ allele in E17.5 heterozygotes (A, D), followed by immunostaining of cytokeratin (B, C) or vimentin (E, F), showing that X-gal positive cells were present in both cytokeratin-positive cells (arrows in B and C) and vimentin-positive cells (arrows in E and F). Panels C and F are digitally enlarged from portions of panel B and E (shown as rectangles) for visualization purpose. Only a few X-gal positive cells (arrow heads in B) were seen in the proximal airways (B). The X-gal positive signals (blue in A, D) was pseudo-colorized to blue (B, C) and green (E, F) for the ease in visualization. Br: bronchus.