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Serial dilutions of JURKAT cells expressing Vβ-family 8 spiked in PBMCs of three healthy volunteers. (A) measured percentages of family 8 in case of 50, 500, 5000, 50 000 JURKAT cells added to 100 000 PBMCs; (B) mean percentages of the three volunteers compared with theoretical values calculated assuming 5.88% family 8 without JURKAT cells and 70% TCR α/β positive cells in 100 000 PBMCs. Pj: Vβ-chain relative expression.
