Table 2.
Primers/probes sets for quantification of PBGD and Cα-chain (FRET).
| Primer/probe | Sequence (5'-3') | TA | MgCl2 (mM) |
Standard dilutions (pg/μl) |
| PBGD-fwd | TGC AGG CTA CCA TCC ATG TCC CTG C | 65°C | 4 | 1/0.1/0.001 |
| PBGD rev | AGC TGC CGT GCA ACA TCC AGG ATG T | |||
| PBGD-3Fl | CGT GGA ATG TTA CGA GCA GCA GTG ATG CCT ACC-Fl | |||
| PBGD-5LC | LC-TGT GGG TCA TCC TCA GGG CCA TCT TC-ph | |||
| HAC2-fwd | ACA CCT TCT TCC CCA G | 60°C | 3 | 10/1/0.01 |
| HAC2-rev | TCC AGT TGG TGG CAT T | |||
| HAC2-3Fl | GTG ATT GGG TTC CGA ATC CTC C-Fl | |||
| HAC2-5LC | LC-CTG AAA GTG GCC GGG TTT AAT CT-ph |
TA: Annealing temparature; Fl: Fluorescine; LC: LC Red 640; ph: phosphate