Table 2.
Primers and conditions used for PPOX gene and cDNA amplification
| Amplified region (exon) | Amplified Region (intron) | MW (pb) | Sequence (5' to 3') | Annealing (°C) | Mg+2 (mM) | |
| I | 2, 3, 4 | 2, 3 | 758 | F: GCTTCTGGAGCGCAGGTTGTCC R: AAGGCATATGAGGATGAGGGCA |
60 | 1.6 |
| II | 5, 6 | 5 | 680 | F: AGGTATGTCAGGAGCTTCCCCC R: CCCTCACTTTGGCAGTACTTAA |
60 | 1.6 |
| III | 7, 8 | 7 | 852 | F: TGCTGGGATTACAGGTGT R: AGCTTTTGCTTCTCACTGGTAGG |
62 | 2.5 |
| IV | 9 | ----------- | 321 | F: GATTACAGGTGTGAGCCACCA R: CCTACCAGTGAGAAGCAAAAGCT |
60 | 2 |
| V | 10, 11,12, 13 | 10,11,12 13 | 859 | F: GCCCTTTCCTTCTGACGCATG R: GCCAGACCAAGCCAAGCCAAGC |
62 | 2.5 |
| VI | 1 | 1 | 440 | F: CCAAGTCCCGCCAATCCAGAT R: GGACAACCTGCGCTCCAGAAGC |
60 | 5 |
| VII | Promotor | ----------- | 705 | F: AGGTGATAGAGAACTGGCCCAA R: CAGCCTTTTCGGTCTCTCCTA |
63 | 3 |
| VIII | ------------ | 4 | 786 | F: TCTGAGCTTGGCTTGGATTC R: CTCTGCAGCTGGTTTAGG |
60 | 2 |
| IX | ------------ | 6 | 528 | F: GCTTTCCCAGTCTCTTCC R: AAGGCCTGGCGAATGAGT |
60 | 2 |
| X | ------------ | 8 | 313 | F: ATTCTCATTTTCTGGGTCTCTC R: TCAGCAGGGAGCAGCTCACTG |
60 | 2 |
| XI | ------------ | 9 | 535 | F: CTGAGTGCCATCACTGCA R: GCTCAGGGAAAGCAACTG |
60 | 2 |
| XII | Gen | All | 5,500 | F: AGAGAACTGGCCCAAAATTGGAGT R: CAGACCAAGCCAAGCCAAGCAATT |
60 | 2 |
| 4–9 | From nt 226 (exon 4) to nt 893 (exon 9). | 668 | F: TCTGAGCTTGGCTTGGATTC R: GTCACTCGACGAGGGACGACT |
60 | 3 | |
| 6–9 | From nt 478 (exon 6) to nt 893 (exon 9). | 416 | F: TCTCTAGCCATGGACAGTCT R: GTCACTCGACGAGGGACGACT |
60 | 6 | |
All primers were developed in the course of this study. Primers I to XII were used for DNA amplification and primers 4–9 and 6–9 were used for cDNA amplification.