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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Mol Genet Metab. 2008 Mar 17;94(2):222–233. doi: 10.1016/j.ymgme.2008.01.014

Figure 1. Schematic of the generation of the CLN2 hypomorph.

Figure 1

Panel A, The CLN2 targeted allele was originally generated by the synergistic combination of an Arg446His allele with the insertion of a neomycin selection cassette into intron 10 of the murine CLN2 gene which results in an unstable mRNA containing a ~ 500 nt reverse orientation neo insertion [9]. The targeted CLN2 alleles described in this study were generated by the cre-mediated excision of the neo marker allowing for transcription of a normally spliced and stable CLN2 mRNA that differs from the wild-type transcript only in the presence of the missense mutation. Panel B, Details of the targeted CLN2 allele and genotyping strategy.