. Author manuscript; available in PMC: 2008 Jul 16.

Published in final edited form as: Dev Biol. 2007 May 1;307(2):202–213. doi: 10.1016/j.ydbio.2007.04.029

Table 3.

Partial knockdown of loxl1 or loxl5b sensitizes embryos to notochord distortion in the presence of suboptimal copper nutrition. Wild-type embryos were injected with control or lysyl oxidase-specific morpholino, as indicated, and incubated with or without neocuproine at doses that were determined not to cause notochord distortion alone. Embryos injected with lysyl oxidase-specific morpholino and incubated in 2 μM neocuproine were sensitized to develop notochord distortion. The number of embryos examined includes dead embryos that could not be scored for notochord phenotype. Each result is pooled from three independent experiments, which were scored at 24 hpf.

Specific Morpholino	Dose of morpholino (ng)	Pharmacologic treatment	# of embryos examined	Phenotype of notochord distortion
Specific Morpholino	Dose of morpholino (ng)	Pharmacologic treatment	# of embryos examined	−	+
Ctrl	2.4	None	104	104 (100%)	0 (0%)
Ctrl	2.4	2 μM neocuproine	113	111 (98%)	2 (2%)
*loxl1*	2.4	None	143	127 (100%)	0 (0%)
*loxl1*	2.4	2 μM neocuproine	132	49 (39%)	77 (61%^*)
Ctrl	5	None	139	136 (100%)	0 (0%)
Ctrl	5	2 μM neocuproine	138	132 (99%)	2 (1%)
*loxl5b*	5	None	147	126 (95%)	7 (5%)
*loxl5b*	5	2 μM neocuproine	147	1 (1%)	140 (99%^*)

p < 0.01 versus controls by ANOVA