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. 2008 May 20;33(6):531–539. doi: 10.1093/chemse/bjn021

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© 2008 The Authors

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Double-label immunohistochemistry (IHC) for β-gal in green and either tyrosine hydroxylase (TH, A through F) or GAD67 (G through L) in red in the OB of BDNF^lacZneo heterozygous mice. TH and GAD67 are markers for 2 different populations of PG cells. (A, D) β-gal IHC (green). (B, E) Corresponding TH IHC (red). (C, F) Combined β-gal/TH images. Square in (C) indicates region shown in (D) to (F). (G, J) β-gal IHC (green). (H, K) Corresponding GAD67 IHC (red). (I, L) Combined β-gal/GAD67 images. Arrows indicate β-gal-negative/GAD67-positive cells. Square in (C) indicates region shown in (D) to (F). Scale bar: 20 μm.