Table 2.
Primary and secondary antibodies
Fluorophore | Target | Host | Concentration | Source |
CGRP | Rabbit | 1:1000 | Peninsula Laboratories | |
β-gal | Guinea pig | 1:1000 | Drs Tom Finger and Cindy Yee (Yee et al. 2003) | |
β-gal | Rabbit | 1:1000 | Cappel | |
GAD67 | Rabbit | 1:1000 | Chemicon | |
GFAP | Mouse | 1:400 | Sigma-Aldrich | |
PGP 9.5 | Rabbit | 1:1000 | Biogenesis | |
TH | Rabbit | 1:2000 | Calbiochem | |
Fluorescein Isothiocyanate (FITC) | Chicken IgY | Donkey | 1:200 | Jackson Laboratories |
Rhodamine | Chicken IgY | Donkey | 1:200 | Jackson Laboratories |
Rhodamine | Goat | Donkey | 1:200 | Jackson Laboratories |
Alexa 488 | Goat | Donkey | 1:200 | Molecular Probes |
Rhodamine | Guinea pig | Donkey | 1:200 | Jackson Laboratories |
Alexa 488 | Guinea pig | Goat | 1:200 | Molecular Probes |
Alexa 568 | Mouse | Goat | 1:200 | Molecular Probes |
Alexa 488 | Rabbit | Goat | 1:200 | Molecular Probes |
Alexa 568 | Rabbit | Goat | 1:200 | Molecular Probes |
TH, tyrosine hydroxylase.