Figure 4.
Western blot of POMC and 23 kDa and 21 kDa immunoreactive ACTH in secretion medium. (A) Western blot showing POMC and 21–23 kDa ACTH in the secretion medium from anterior pituitary cells of normal and Cpefat mice, with and without CRH treatment. Molecular mass markers are on the left, and the forms of ACTH are indicated by arrows on the right. (B) Western blots similar to that shown in A were quantitated. The sum of these three forms of ACTH from four Western blots of control (without CRH) secretion medium were averaged and made to equal 100% for normal and Cpefat animals (open bars). The sum of the three forms of ACTH in the secretion medium with CRH treatment was expressed as a percentage of control for normal and Cpefat mice. The mean percentage of control ± SEM (n = 4) is shown (solid bars). No significant difference was found between control and CRH treatment for both normal and Cpefat animals.