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. 2008 Apr 19;64(Pt 5):525–531. doi: 10.1107/S0907444908004277

Table 1. Crystallization conditions.

Crystal Protein concentration (mg ml−1) Reservoir solution
A 40 15% PEG 400, 0.8 M NaCl, 50 mM Li2SO4 in 50 mM Tris–HCl pH 8.0
B 5 4% PEG 4000, 50 mM KCl, 25% ethylene glycol (EG) in 50 mM Tris–HCl pH 7.0
C 5 4% PEG 2000, 50 mM KCl, 25% EG in 50 mM Tris–HCl pH 7.0
D 40 2.4% PEG 4000, 0.8 M NaCl, 50 mM Li2SO4 in 50 mM Tris–HCl pH 8.0
E 5 4% PEG 3350, 50 mM ammonium acetate, 25% EG
F 5 3% PEG 8000, 50 mM KCl, 25% EG in 50 mM Tris–HCl pH 7.0

Vault was dissolved in buffer containing 50 mM Tris–HCl pH 7.5, 75 mM NaCl and 1.5 mM MgCl2. The crystallization temperature for crystals AD and E was 288 K and that for crystals BC and F was 277 K.