Table 1. Crystallization conditions.
| Crystal | Protein concentration† (mg ml−1) | Reservoir solution |
|---|---|---|
| A | 40 | 15% PEG 400, 0.8 M NaCl, 50 mM Li2SO4 in 50 mM Tris–HCl pH 8.0 |
| B | 5 | 4% PEG 4000, 50 mM KCl, 25% ethylene glycol (EG) in 50 mM Tris–HCl pH 7.0 |
| C | 5 | 4% PEG 2000, 50 mM KCl, 25% EG in 50 mM Tris–HCl pH 7.0 |
| D | 40 | 2.4% PEG 4000, 0.8 M NaCl, 50 mM Li2SO4 in 50 mM Tris–HCl pH 8.0 |
| E | 5 | 4% PEG 3350, 50 mM ammonium acetate, 25% EG |
| F | 5 | 3% PEG 8000, 50 mM KCl, 25% EG in 50 mM Tris–HCl pH 7.0 |
†
Vault was dissolved in buffer containing 50 mM Tris–HCl pH 7.5, 75 mM NaCl and 1.5 mM MgCl2. The crystallization temperature for crystals A, D and E was 288 K and that for crystals B, C and F was 277 K.