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. 1997 May 13;94(10):5355–5360. doi: 10.1073/pnas.94.10.5355

Figure 1.

Figure 1

(A) Western blot analysis of HES-1 protein in lung cancer cell lines, grouped by hASH1 mRNA expression status. Extracts from 1 × 106 cells separated by SDS/PAGE were analyzed using polyclonal rabbit antisera raised against a C-terminal peptide common to rat and human HES-1. (B) Lysates (5 μl of 50 μl) from in vitro translation reactions containing no plasmid (control) or a HES-1 expression construct, as well as an extract from NCI-H157 cells, were analyzed for HES-1 protein by Western blotting as above.