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. 1997 May 13;94(10):5355–5360. doi: 10.1073/pnas.94.10.5355

Figure 4.

Figure 4

Gel mobility-shift analysis of the hASH1 promoter class C site. (A) Cell-type-specific shift patterns. Nuclear protein extracts (4 μg) from native cells (lanes 2–5) or from DMS53 cells expressing HES-1 (lane 9) or control constructs (lanes 6–8) were incubated with a WT probe, containing the class C site from the hASH1 promoter. (B) Effect of HES-1 antisera on complexes with the hASH1 promoter class C site. NCI-H157 nuclear extracts (4 μg) and various antisera were preincubated for 30 min, then incubated with WT probe. Lanes: 1, free probe; 2, extract with buffer alone, no antisera; 3, preimmune sera; 4, HES-1 antisera; 5, control hASH1 antisera.