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. 1997 May 13;94(10):5355–5360. doi: 10.1073/pnas.94.10.5355

Figure 5.

Figure 5

(A) Oligonucleotide sequences used for gel mobility-shift analysis: WT, derived from the hASH1 promoter (base pairs −265 to −247) containing the class C site (underlined); mutant, identical to WT except for five point mutations in the class C site (underlined); CT7–8, an irrelevant probe derived from the calcitonin gene promoter (38); and 2NB, containing the two N-box HES-1 binding sites (underlined) in the rat HES-1 promoter (27). (B) Competitor studies of the hASH1 promoter class C site using NCI-H157 nuclear protein extracts (4 μg), WT probe, and competitors shown in A. Lanes: 1, free probe; 2, no competitor; 3 and 4, 50- and 150-fold excess WT; 5 and 6, mutant; 7 and 8, CT7–8; 9 and 10, 2NB.