Fig. 1.

Chloroplast transformation with the FokI methylase. The transformation vector pFaadMet2 targets transgene insertion to the small single copy region at a site proximal to the ndhF gene (a). Insertion at this site results in an increase in size of 3,278 bp for the BglII fragment containing the ndhF probe sequence (b). Following Southern analysis, the absence of the smaller band derived from the wild type (WT) plastome indicates that all six transplastomic plants are homoplastomic (lanes 1–6)