Table 1.
Results of mosaicism by H19 methylation Southern blots and IFG-II SNuPE assay in normal kidney and blood samples of Wilms tumour patients
| Sources | Ratio of H19 methylation,* mean ± SD | % mosaicism† (H19 Southern blots) | % mosaicism‡ (IFG-II SNuPE) |
|---|---|---|---|
| Adult normal kidney | 1.01 ± 0.06 [10] | ||
| NonLOI, nonLOH kidney | 0.97 ± 0.05 [8] | 0 | 0 [6] |
| LOI kidney | |||
| Case 1 | 3.59 ± 0.37 (3) | 56 | 76 |
| Case 2 | 5.40 ± 0.25 (3) | 69 | 85 |
| Case 3 | 3.36 ± 0.32 (3) | 54 | 66 |
| Case 4 | 2.34 ± 0.24 (3) | 40 | NI |
| Case 5 | 2.20 ± 0.15 (2) | 38 | 23 |
| Case 6 | 1.45 ± 0.18 (3) | 18 | 26 |
| Case 7 | 1.15 ± 0.07 (3) | 7 | 6 |
| Case 8 | 1.29 ± 0.10 (2) | 13 | 9 |
| LOH kidney | |||
| Case 9 | 3.52 ± 0.13 (3) | 56 | NA |
| Cases 10–15 | 1.04 ± 0.07 (2) | <5 | NA |
| Normal blood | 0.99 ± 0.06 [4] | 0 [3] | |
| Blood from nonLOI, nonLOH | 0.90 ± 0.09 [6] | 0 | § |
| Blood from LOI | |||
| Case 1 | 0.92 ± 0.08 (2) | 0 | 0 |
| Case 2 | 2.43 ± 0.21 (3) | 42 | 32 |
| Case 3 | 0.98 ± 0.07 (2) | 0 | 0 |
| Case 6 | 0.89 ± 0.07 (2) | 0 | § |
| Blood from LOH | |||
| Case 9 | 0.95 ± 0.10 (2) | 0 | NA |
| Cases 10–13 | 0.88 ± 0.14 (2) | 0 | NA |
The number of cases examined is shown in square brackets. The number of independent experiments analyzed for each case is shown in parentheses. NI, not informative at the exon 9 ApaI polymorphism; NA, not applicable. The ratios of intensities between methylated and unmethylated alleles are summarized. The average of the ratio of normal individual cases was standardized to 1.00 in each blot.
Proportion of cells with H19 methylated biallelically.
Proportion of cells with biallelic IFG-II expression. Cases which are polymorphic at the exon 9 ApaI site were used. Each sample was analyzed by two or more independent SNuPE assays. The mean value is shown for each case.
Samples were not available.