Skip to main content
. 1997 May 13;94(10):5372–5377. doi: 10.1073/pnas.94.10.5372

Figure 1.

Figure 1

Reduction of commercial wheat allergen extract with the NADP/thioredoxin system determined by SDS/PAGE/mBBr labeling procedure. After incubation with the indicated additions, the wheat proteins were derivatized with mBBr, and fluorescence was visualized after SDS/PAGE. Seventy micrograms of commercial wheat allergen extract was applied to all lanes, except lane 1, in 30 mM Tris⋅HCl, pH 7.9. Lane 1, NTS alone: NADPH, NTR, and thioredoxin (both from E. coli). Lane 2, DTT, heat: the sample was heated 5 min in boiling water in the presence of DTT. Lane 3, DTT/Trx: DTT and thioredoxin (from E. coli). Lane 4, NGS: NADPH, glutathione, glutathione reductase (from spinach leaves). Lane 5, NTS: NADPH, NTR, and thioredoxin (both from E. coli). Lane 6, control: no addition. Note that the excess NADPH and DTT maintained NTR, thioredoxin, and target allergen proteins in the reduced state throughout the experiment.