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. 1997 May 13;94(10):5378–5382. doi: 10.1073/pnas.94.10.5378

Figure 1.

Figure 1

SfiI-NotI-I-CeuI map of the B. subtilis 168 genome. The physical map of the 4,188-kb B. subtilis genome for NotI (inner circle), SfiI (outer circle), and I-CeuI map (equivalent of the rrn map) is shown. The sizes of the I-CeuI fragments >100 kb are shown by arcs with two arrowheads. The loci of oriC and terC have been determined (14, 18). Strain BEST4173 has the [ne] cassette at 1,255 kb, the [eo] cassette at 1,551 kb, and the oriN (closed circle) at 1,375 kb of the physical map as described in the materials and methods section. The spectinomycin resistance gene (spc) included in the [ne] cassette and the blasticidin S resistance gene (bsr) in the [eo] cassette are shown. The 300-kb DNA segment (1,255–1,551 kb) is shown by double arcs.