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Chaperonins stored for >48 h require Mg2+ and nucleotides to form filaments at a physiological temperature. (A) “Aged” chaperonin (1 mg/ml) in Hepes buffer (pH 7.5) and 25 mM MgCl2 after 1 h at 75°C; (B) the same chaperonin sample with 1 mM ATP added; (C) with 1 mM ADP added; and (D) with 1 mM AMP-PNP added.
