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. 1997 May 13;94(10):5395–5400. doi: 10.1073/pnas.94.10.5395

Figure 2.

Figure 2

A representative sucrose gradient-derived dot blot probed with oligonucleotides designed from human FMR1 (nucleotides 118–162) shows that FMR1 mRNA moves into small polyribosomes shortly after mGluR stimulation by 10−2 M DHPG; unstimulated and stimulated aliquots were processed in parallel. Abscissa: dots from serial samples, starting at the top of a 15–45% continuous sucrose gradient. Ordinate: pixel density of dot image, measured on a Fujix BAS 1000 phosphorimager. The original dot images are shown at the base of the figure, along with a tracing of the OD260 polysomal profile.