Figure 4.

Block of coimmunoprecipitation of native synaptotagmin with syntaxin by synprint peptide. Concentration-dependent inhibition of the association of native synaptotagmin with syntaxin by the synprint peptide His-L_II–III(718–963). The complex of syntaxin with synaptotagmin was coimmunoprecipitated from solubilized rat brain synaptosomes with a monoclonal anti-syntaxin antibody (10H5) in the presence or absence of an increasing amount of His-L_II–III(718–963), as indicated, or in the presence of 1.7 μM of a control fusion protein, L_II–III(670–800), from the rat skeletal muscle L-type calcium channel α_1S.