Figure 3.

Effects of 8Br-cAMP on cell-associated APP and GFAP from cultured astrocytes. Confluent astrocytes were treated with 8Br-cAMP for 24 h. (A) Representative immunoblot with R37 antiserum and GFAP mAb revealed increases in APP (≈120 kDa) and GFAP (≈50 kDa), respectively, with increasing concentrations of 8Br-cAMP. Similar results were obtained from three independent experiments. (B) Treatment for 24 h with increasing concentrations of 8Br-cAMP or forskolin (50, 100, or 250 μM) increased the amounts of cell-associated APP, as detected by antiserum R37 or mAb 22C11 (∗, P < 0.05). The graph represents data accumulated from three independent experiments.