Figure 4.

Effect of overexpression of a CyPA mutant [CyPA(R55A)] deficient in PPIase activity on the ability of CsA to reduce functional α7 receptor expression. The open bars represent CsA-treated oocytes [n = 5 for α7/CyPA injected oocytes and for α7/CyPA(R55A) injected oocytes]. The solid bars represent vehicle-treated oocytes [n = 5 for α7/CyPA injected oocytes and for α7/CyPA(R55A) injected oocytes]. The ratio of cDNAs in each coinjection was 1:1. Both CyPA and CyPA(R55A) used in this experiment possess a C-terminal hexahistidine tag that was used to verify their expression by nickel affinity matrix purification.