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. 1997 May 13;94(10):5432–5437. doi: 10.1073/pnas.94.10.5432

Figure 5.

Figure 5

Western blot of hexahistidine-tagged CyPA(R55A) from Xenopus oocytes obtained by Ni-NTA matrix purification. Lanes SM, W, E, and C represent starting material, 20 mM imidazole wash, 500 mM imidazole eluate, and flow through from CsA-affinity matrix purification, respectively. (Upper) CyPA(R55A)his-expressing oocytes; (Lower) wild-type CyPA-expressing oocytes. Immunoreactivity to a CyPA anti-peptide antibody is indicated by an ≈18-kDa band (arrow) seen only in the starting material and the 500 mM imidazole eluate.