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. 1997 May 13;94(10):5444–5449. doi: 10.1073/pnas.94.10.5444

Figure 1.

Figure 1

Generation and structural characterization of the recombinant adenovirus vector AdCMVssTnI. (A) Scheme for creating the recombinant adenovirus AdCMVssTnI with plasmid pJM17 and plasmid pAdCMVssTnI, which contains the rat ssTnI expression cassette. (B) Recombinant AdCMVssTnI was identified by Southern blot analysis of viral lysates. Lane 1, undigested recombinant AdCMVssTnI DNA; lane 2, BglII digest, and lane 3, BamHI digest. The BglII excised the expected 1,400-bp fragment, containing the expression cassette and the left end of the adenovirus genome, whereas digestion with BamHI excised the full-length ssTnI cDNA of predicted size (800 bp).