Figure 1.

Immunoblots of membrane fractions (20 μg/lane) from total rat kidney prepared from sham-operated rats (S) and experimental rats with CHF. (A) The immunoblot was reacted with affinity purified anti-AQP2 and reveal 29-kDa and 35- to 50-kDa AQP2 bands. A more prominent labeling is seen in CHF rat kidneys. (B) Immunoblotting control. (C) Densitometry performed on all sham-operated animals (n = 14) and all animals with severe CHF (n = 12). A highly significant increase in AQP2 expression is seen in CHF animals.