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. 1997 May 13;94(10):5461–5466. doi: 10.1073/pnas.94.10.5461

Figure 5.

Figure 5

Western blot analysis of AEOMT. Lanes: 1, total protein (15 μg) from E. coli containing vector only (E. coli control); 2, purified insoluble AEOMT fusion protein (0.5 μg) from E. coli expressing AEOMT cDNA, with an approximate size of 43 kDa; 3, total soluble protein (30 μg) from yeast containing vector only (yeast control); 4, AEOMT protein (≈42 kDa) in total soluble protein (30 μg) from yeast expressing AEOMT cDNA; 5, AEOMT protein (≈42 kDa) in total soluble protein (14 μg) from secondary developing xylem of loblolly pine. Molecular mass markers are to the left.