Fig. 1.

Bar graph of the TUNEL-positive apoptotic cells and G2/M phase cells before and after UV irradiation. TUNEL-positive cells: UACC903 and UACC903(+6) cells before and at 12 h after the UV irradiation were processed by the terminal deoxynucleotidyl transferase-mediated dUTP biotin nick-end labeling (TUNEL) assay using the In Situ Cell Death Detection Kit (TMR, Roche). On average, 700 cells were analyzed for each of triplicate experiments to calculate means and SD of the TUNEL-positive cells. G2/M-phase cells: UACC903 and UACC903(+6) cells were sorted based on DNA contents after staining, with propidium iodide (PI), UACC903 and UACC903(+6) cells before and at 12 h after the UV irradiation. 10,000 cells were sorted using ELITE Flow Cytometry (Beckman Coulter) and analyzed with Cell Quest software (Becton Dickinson). Means and SD were calculated from triplicate experiments. The P-values between comparisons are indicated. 903: UACC903, 9036: UACC903(+6), 903UV: UACC903 treated with UV, 9036UV: UACC903(+6) treated with UV