Figure 1.

Physical interaction between TR4 and AR. (A) The GST-TR4 fusion protein and GST control protein were purified as instructed by the manufacturer (Amersham Pharmacia). Five microliters of in vitro-translated ³⁵S methionine-labeled AR, TR2, and RXRα was incubated with the GST-TR4 or GST bound to glutathione-Sepharose beads in a pull-down assay as described. (B) Localization of the interaction domain within AR. Different AR deletion mutants, AR-N, AR-D, and AR-L, were in vitro-translated and incubated with the GST-TR4 in a pull-down assay. The input represents 20% of the amount of labeled protein used in the pull-down assay. The pull-down complex was loaded onto an 8% or 15% polyacrylamide gel and visualized by autoradiography.