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Superose-12 gel permeation chromatography with fluorescence detection of culture medium containing (Ser-Hyp)32-EGFP (A), (GAGP)3-EGFP medium concentrated 4-fold (B), medium of EGFP targeted to the extracellular matrix concentrated 10-fold (C), or 10 μg of standard EGFP from CLONTECH (D). Not shown is the fractionation of medium from nontransformed tobacco cells, which gave no fluorescent peaks, consistent with the results presented in Fig. 2C. LU, luminescence units.
