Figure 4.
PRP-1 reverse-phase fractionation of the Superose-12 peaks containing (Ser-Hyp)32-EGFP (A), (GAGP)3-EGFP (B), and (glyco)proteins in the medium of nontransformed tobacco cells (C). Endogenous tobacco AGPs eluted between 47 and 63 min; extensins eluted at ≈67 min. (C) Control medium collected from nontransformed tobacco cells was first fractionated on Superose-12, and the fractions eluting between 47 and 63 min were collected for further separation on PRP-1 to determine whether any endogenous AGPs/HRGPs cochromatographed with (Ser-Hyp)32-EGFP or with (GAGP)3-EGFP, which they did not.