Figure 3.

Superposition of Sec18p-N (pink) and NSF-N (purple). Sec18p-N (residues 26–61, 64–105, 111–125, 133–178, and 194–210) was aligned on NSF-N (residues 3–38, 46–86, 89–103, 111–156, and 173–189). The N_A and N_B subdomains are indicated, and structurally divergent regions are labeled. “Sec18p N-term ext” refers to a 19-aa extension at the N terminus of Sec18p that is lacking in mammalian NSF and was deleted from the Sec18p-N protein expression construct. Figure was generated with molscript (36).