Table 1.

Nucleotide sequence, restriction sites, and purpose of all synthesized primers

Primer	5′-nucleic acid sequence-3′
P1  for 20 mer, 1536× degeneracy	ACN ATH TTY YTN GGN TAY AC
P2  for 19 mer, 1024× degeneracy	GN TAY YTN GTN CAR CAY AA
P3  for 18 mer, 1024× degeneracy	GGN GGN MTN GAR GAR GAY
P4  rev 18 mer, 384× degeneracy	CAY CAY ATH GCN AAY GCN
P5  rev 19 mer, 1024× degeneracy	TA NTC NGC NCC RTT NCK CA
P6  rev 18 mer, 1024× degeneracy	CCA NSW NAC NGC YTC RTC
P7  for 3′RACE (HSS)	CGA GAC TTC ATA TCT GTA TTG GGC ATA TAA
P8  for 3′RACE (HSS) (nested)	AAC AGA TGG CTC TAT CGG AGA CAT GTT
P9  rev 5′RACE (HSS)	CCC TTT CGA TCT CAA AT
P10 rev 5′RACE (HSS) (nested 1)	TCT CAA ATC AGC TCC GGG TAA AGA A
P11 for 5′RACE (HSS) (nested 2)	GTG CTT GCT AGG CAT TTT ATG A
P12 for 3′RACE (DHS)	GAA TTG CGT TCG AAA GGA CTA AAT CGT ATT
P13 rev 5′RACE (DHS)	CGC ATT ATT AGT TTT GAT
P14 rev 5′RACE (DHS) (nested 1)	TTT GTT CTT CCA ACA CTT GGT CAA ATA
P15 rev 5′RACE (DHS) (nested 2)	GTT GTC ATT CGG CAC CAA CAA
P16 for full-length (HSS), NdeI	TA TAT CAT ATG GCC GAG TCA AAC AAA GAA G
P17 rev full-length (HSS)	TT ACC TGA TCA AAA ACC ATT GAC TTT AGA TGC
P18 for full-length (DHS)	TA TCT AGA ATG GAA GAA TCT ATG AAA CAA G
P19 rev full-length (DHS), BamHI	TAA GGA TCC TCA GGA ACT CGG CTC AGC
P20 for 20 mer, 64× degeneracy	ATG TCN GAY GAR GAR CAY CA
P21 for full-length (eifsv), NdeI	TA TAT CAT ATG TCG GAT GAG GAG CAC CA
P22 rev full-length (eifsv), XhoI	TAA CTC GAG TTT TGG GCC AAT ATC CTT AAG AG

For the degenerated primers P1–P6 and P20 the following code was used: K = G + T, H = A + T + C, M = A + C, N = A + T + C + G, R = A + G, S = C + G, W = A + T, Y = T + C. Restriction sites are underlined and the start-ATG is shown in bold; for, forward primer; rev, reverse primer.