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. 1999 Dec 21;96(26):14783–14788. doi: 10.1073/pnas.96.26.14783

Figure 5.

Figure 5

Properties of the mthMCM DNA helicase activity. (A) NTP requirement for mthMCM helicase activity. DNA helicase activity assays were performed with the indicated amounts of mthMCM protein (multimer, glycerol gradient fraction peak) and 10 fmol of HS-3′T substrate in the absence or presence of 5 mM rNTPs or dNTPs. (B) mthMCM helicase translocates in the 3′-to-5′ direction. DNA helicase activity was assayed with 10 fmol of HS-M, HS-5′T, or HS-3′T and various amounts of the mthMCM multimeric form (glycerol gradient peaks). (C) Effect of SSBs on the mthMCM helicase activity. DNA helicase assays were performed with 5 fmol of HS-3′T substrate. The amounts of E. coli SSB and mthRPA added to reactions were as indicated.