Figure 1.
Effect of MalE-MinC fusions on cell division. (A and B) The MalE-MinC fusion blocks cell division and Z ring formation. A culture of JS964 (Δmin) pZH101 (PBAD∷malE-minC) was processed for immunofluorescence microscopy before (A) and 1 hr after (B) addition of 0.01% arabinose. The cells were immunostained with antibodies to FtsZ, and a secondary antibody was conjugated to the fluorophore Cy3. The panels to the left are phase contrast micrographs, and those to the right are fluorescence micrographs. (C) A MalE-MinC19 fusion is attenuated for inhibition of cell division. JS964 (Δmin) pZH102 (PBAD∷malE-minC19) was induced with 0.01% arabinose for 1 hr, and a sample was taken and immunostained for FtsZ.