Figure 2.

Monitoring interactions between FtsZ and MalE-MinC fusions by using an optical biosensor. Biotinylated FtsZ was immobilized in a biotin cuvette as described in Materials and Methods. MalE-MinC or MalE-MinC19 was added at the concentrations indicated in a final volume of 60 μl, and the response in arc seconds was measured versus time (Upper). The data obtained from these experiments were analyzed with iasys fastfit software by using single phase association to obtain the equilibrium binding plots and K_d values (Lower).