Figure 5.

MalE-MinC19 has reduced inhibitory activity in the FtsZ assembly assay. FtsZ was incubated with increasing amounts of MalE-MinC19 in polymerization buffer. Assembly was initiated with 1 mM GTP, and samples were centrifuged and were analyzed by SDS/PAGE. The amount of FtsZ in the pellet was quantitated and plotted along with the results obtained with MalE and MalE-MinC additions (data from Fig. 4). The amount of FtsZ in the pellet in the absence of MalE or the fusions was set at 100%. This represents ≈50% of the FtsZ in the reaction (9, 42).